1	The biodiversity and evolutionary relationships of the Australian members of the liverwort genus Fossombronia through molecular sequences Melita Baum Supervisor: Dr D Christine Cargill
2	Bryophytes Liverworts
3	Liverworts
4	Liverworts
5	Fossombronia Fossombroniaceae Austrofossombronia Fossombronia
6	Systematics The study of biological diversity and its evolutionary history The development of molecular techniques has transformed systematics
7	Bryophyte systematics
8	The project Diversity of Fossombronia within Australia is poorly known Current Australian Fossombronia collections have been identified based on morphology, particularly of spores Is spore morphology accurate for determining species?
9	Morphology - gametophyte
10	Morphology - spores
11	Methods – material used All plant samples from culture Collections from across Australia Sequences for overseas and outgroup species from Genbank
12	Markers Nuclear region ITS (internal transcribed spacer) Chloroplast region rps4 trnL/F
13	Methods Extraction Polymerase chain reaction (PCR) Clean up Sequencing Alignment of sequences
14	Analysis Parsimony analysis ILD test Model test HKY + I + G Bayesian analysis
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22	Summary High diversity of Fossombronia exists in Australia Possible rapid radiation Paraphyletic
23	Acknowledgments Christine Cargill Ish Sharma Bernard Pfeil Crandall-Stotler/ Stotler Labs, USA CSIRO Plant Industries