Born in Melbourne, the youngest of four children, to a clerk, Henry Brotherton, and Jane née Hodgkins (c.1835-1916). The family moved to the residence 'Woodlands' at Castlemaine after Anna's father retired in 1887.
Anna collected MEL specimens at Barkers Creek, Loddon River, Castlemaine, and summit of Mount Alexander, 1894-1895. She was honorary secretary of the Castlemaine Field Rambler's Club. M was acquainted with Anna's father. Anna did not marry, and died in 1956 at the age of 82.
Three sheets of specimen identifications in Mueller's hand, an envelope addressed by Mueller to Anna, and two letters from Mueller to Anna survive.
Source: extracted from: Maroske, Sara and Vaughan, Alison (2014) 'Ferdinand Mueller's female plant collectors: a biographical register', Muelleria Vol.32 [consult for source references]